High solid polyurethane coatings (PUR) with high gloss and low surface roughness were obtained using bio-polyols synthesized from soybean oil (BPS), linseed oil (BPL) and rapeseed oil (BPR), polyisocyanates derived from isophorone diisocyanate (IPDI), trimethyl-1,6-hexamethylene diisocyanate (TMDI) or dicyclohexylmethane-4,4’-diisocyanate (HMDI) and xylene as a solvent. All the obtained PUR coatings contained about 40 % bio-based components and showed good mechanical properties. The characterization of PUR coatings was carried out on the basis of the determination of roughness, surface free energy (SEP) and mechanical properties such as relative hardness, cupping ability, adhesion, scratch, and impact resistance. The biotic degradation behavior in liquid or inert solid media and abiotic degradation behavior (in water, sea water, brine, thermal shock) were analyzed. It has been found that the obtained PUR coatings exhibit excellent resistance to corrosion. Also, they are not susceptible to enzyme-catalyzed hydrolysis. Studies showed that the obtained coatings hold promise for use as effective protection of steel surfaces.