Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants

W. Andrzejewska; Z. Pietralik; M. Taube; A. Skrzypczak; M. Kozak

doi:10.14314/polimery.2014.569

Published : 2014-08-30

Vol. 59 No. 7-8 (2014)

Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants

W. Andrzejewska Z. Pietralik M. Taube A. Skrzypczak M. Kozak

DOI: https://doi.org/10.14314/polimery.2014.569

Abstract

The process of complex formation between cationic gemini surfactants, 3,3'-[a,w-(dioxaalkane)]bis(1-dodecylimidazolium) dichloride, with deoxyribonucleic acid (DNA) was studied. The study was performed for ten surfactants having spacer groups of different lengths used in 6 concentrations (5 mM, 2 mM, 1 mM, 0.5 mM, 0.2 mM, 0.1 mM) and a 6.5 µM DNA solution. The complex formation was verified by circular dichroism spectroscopy and gel electrophoresis. The complexes were found to be stable and the process of complex formation was reproducible, efficient and immediate.

Keywords

gene therapy ; gemini surfactants ; DNA ; circular dichroism ; gel electrophoresis terapia genowa ; surfaktanty gemini ; DNA ; dichroizm kołowy ; elektroforeza żelowa

Details

References

Statistics

Authors

Download files

PDF

Zasady cytowania

Andrzejewska, W., Pietralik, Z., Taube, M., Skrzypczak, A., & Kozak, M. (2014). Structural and spectroscopic studies on the formation of lipoplexes between DNA and cationic gemini surfactants. Polimery, 59(7-8), 569-574. https://doi.org/10.14314/polimery.2014.569

References

Cortesi R., Campioni M., Ravani L., Drechsler M., Pinotti M., Esposito E.: New Biotechnol. 2014, 31, 44,
Crossref

[2] Morille M., Montier T., Legras P., Carmoy N., Brodin P., Pitard B., Benoit J.P., Passirani C.: Biomaterials 2010, 31, 321,
Crossref

[3] Ewert K., Slack N.L., Ahmad A., Evans H.M., Lin A.J., Samuel C.E., Safinya C.R.: Curr. Med. Chem. 2004, 11, 133,
Crossref

[4] Wypych A., Szpotkowski K., Jurga S., Domka L., Kozak M.: Colloid Surf., B Biointerfaces 2013, 108, 212,
Crossref

[5] Porter C.J.H., Pouton C.W., Cuine J.F., CharmanW.N.: Adv. Drug. Deliv. Rev. 2008, 60, 673,
Crossref

[6] Smyth Templeton N.: Curr. Med. Chem. 2003, 10, 1279,
Crossref

[7] Radler J.O., Koltover I., Salditt T., Safynia C.F.: Science 1997, 275, 810,
Crossref

[8] Hait S.K., Moulik S.P.: Curr. Sci. 2002, 82, 1101.

[9] Zana R.: Adv. Colloid Interface Sci. 2002, 97, 205,
Crossref

[10] Kirby A.J., Camilleri P., Engberts J.B.F.N., Feiters M.C., Nolte R.J.M. et al: Angew. Chem. Int. Edit. 2003, 42, 1448,
Crossref.

[11]Wang Ch., Li X., Wettig D., Badea I., Foldvarid M., Verrall R.E.: Phys. Chem. Chem. Phys. 2007, 9, 1616,
Crossref

[12] Piotrowska A., Rybarczyk A., Wierzbicki P., Kotwas M., Wrońska A., Kmieæ Z.: Pol. Ann. Med. 2009, 16, 138.

[13] Piskorska T., Obłąk E.: Postepy Hig. Med. Dosw. (online) 2010, 64, 161.

[14] Schroeder A., Levins C.G., Cortez C., Langer R., Anderson D.G.: J. Intern. Med. 2010, 267, 9,
Crossref

[15] Karlsson L., van Eijk M.C.P., S¨oderman O.: J. Colloid Interface Sci. 2002, 252, 290,
Crossref

[16]Wettig S.D., Badea I., Donkuru M., Verrall R.E., Foldvari M.: J. Gene. Med. 2007, 9, 649,
Crossref

[17] Emel’Yanenko V., Portnova S., Verevkin S., Skrzypczak A., Schubert T. : J. Chem. Thermodyn. 2011, 43, 1500,
Crossref

[18] Zabielska-Matejuk J., Urbanik E., Pernak J.: Holzforschung 2004, 58, 292,
Crossref

[19] Chang Y.-M., Chen C.K.-M., Hou M.-H.: Int. J. Mol. Sci. 2012, 13, 3394,
Crossref

[20] Pietralik Z., Krzysztoñ R., Kida W., Andrzejewska W., Kozak M.: Int. J. Mol. Sci. 2013, 14, 7642,
Crossref

[21] Lee H.K., Mijoviæ J.: Polymer 2009, 50, 881,
Crossref

[22] Luciani P., Bombelli C., Colone M., Giansanti L., Ryhänen S.J., Säily V.M., Mancini G., Kinnunen P.K.: Biomacromolecules 2007, 8, 1999,
Crossref

Google Scholar

Altmetric indicators

Citing articles via

CITEDBY SCOPUS

Statistics

Total abstract views : 79

PDF Downloads : 38

W. Andrzejewska polimery@ichp.pl

Affiliation
Adam Mickiewicz University, Department or Macromolecular Physics, Poznań, Poland

Z. Pietralik

Affiliation
Adam Mickiewicz University, Department or Macromolecular Physics, Poznań, Poland

M. Taube

Affiliation
Adam Mickiewicz University, Department or Macromolecular Physics, Poznań, Poland

A. Skrzypczak

Affiliation
Faculty of Chemical Technology, Poznañ University of Technology, Poznań, Poland

M. Kozak

Affiliation
Adam Mickiewicz University, Department or Macromolecular Physics, Poznań, Poland